Additionally, the purification leads to a reduction of bioburden and endotoxin contamination. This results in a sucrose grade that is low in NPI content, Sucrose Emprove ® Expert. In order to mitigate risks during formulation development, nanoparticulate impurities were removed from sucrose in an improved purification process ( Figure 2). The (1→3)-β-D-glucan levels were measured by using the Glucatell ® Assay (Cape Cod, East Falmout, MA, USA), according to the instructions of the manufacturer. Quantification of β-Glucan contaminationĥ0% sucrose solutions (w/v) were prepared in Milli-Q ® water. DLS data were collected and analyzed with Zetasizer (7.12) software to evaluate sample homogeneity before pooling and concentration for. Nanoparticle Tracking Analysis (NTA) was performed with a NanoSight LM20 (NanoSight, Amesbury, UK) using a pre-run volume of 0.5 mL and triplicate measurements with 0.1 ml sample volume. Amyuni Document Converter 400 Driver Spelunky Hacked Version Nfs Mw 2005 Savegames Stone Hatch For Autocad Media Player Visualizations Windows 10. Samples from size-exclusion chromatography were analyzed by dynamic light scattering (DLS) in a Malvern ZetaSizer Nano series Nano-ZS (model ZEN3600) instrument (Malvern Zetasizer, Worcestershire, UK). The data processing analysis model was set to general purpose. Peak size was based on the viscosity of water as dispersant particle area (%) was based on the intensity. The solutions were measured before and after sterile filtration through a 0.22 μm PVDF membrane.įor Dynamic Light Scattering (DLS), samples were analyzed with the Zetasizer Nano series (Malvern, Herrenberg, Germany) at 25 ☌ using automatic attenuation selection and detection via 173 ° backscatter. Purification of sucrose Particle size analysis and quantificationġ0% sucrose solutions (w/v) were prepared in Milli-Q ® water.
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